Comparative transcriptome analysis of global effect of ddh and lysE deletion on 4-hydroxyisoleucine production in Corynebacterium glutamicum

4-hydroxyisoleucine (4-HIL) is a potential drug for diabetes and weight control. 4-HIL was produced by expressing ido gene in L-isoleucine (Ile)-producing Corynebacterium glutamicum. But L-lysine (Lys) also accumulated as the main by-product this recombinant strain SN02. To attenuate Lys synthesis, two genes synthetic pathway, i.e., ddh encoding diaminopimelic acid dehydrogenase lysE specific exporter were deleted However, deletion of increased titer 28.1%, but did not decrease content; while significantly reduced content 66.7%, decreased 19.3%. Therefore, we carried out transcriptome analysis to reveal global variation these mutants. Deletion (especially lysE) enhanced transcription key enzymes succinylase branch synthesis pathway (DapD DapC) several involved succinyl-CoA accessibility (SucC, SucD OdhI), suggesting compensatory via branch. In addition, ilvBN Ile improved, some 2-methylcitrate cycle inositol metabolism weakened Mere aceA, ppc pck, thus promoting oxaloacetate supply synthesis. affected stress-related transporter genes, that mutant would be under stress, attenuating its These findings will helpful systematic microbiology bio-manufacturing C.